Chemiluminescence Immunoassay (CLIA) is a cutting-edge diagnostic technique that leverages the principles of chemiluminescence and immunoassay to detect and quantify biomolecules with high sensitivity and specificity. In CLIA, an antigen-antibody reaction is utilized to form immune complexes. These complexes are then tagged with a chemiluminescent molecule that emits light when it reacts with a specific substrate.The intensity of this emitted light is directly proportional to the concentration of the target analyte, allowing for precise quantification.
One of the key advantages of CLIA is its enhanced sensitivity, enabling the detection of low-abundance biomarkers that are often missed by traditional methods.This makes CLIA particularly valuable in clinical diagnostics for early disease detection, monitoring disease progression, and assessing treatment efficacy. Its rapid turnaround time and ability to handle multiple samples simultaneously further enhance its utility in high-hroughput laboratory settings.CLIA is widely used in various fields, including endocrinology, oncology, and infectious diseases, to detect hormones, cancer markers, and pathogens, respectively. As advancements in technology continue to refine CLIA's accuracy and efficiency, this technique is set to play an increasingly vital role in modern healthcare, offering a reliable and robust method for disease diagnosis and management.